Part:BBa_K4147002
Expression construct for (CBD)2-DrsB1: Dermaseptin AMP from Phyllomedusa bicolor
This part contains the linear construct for (CBD)2-DrsB1 with an included LacI regulated promoter (1118 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a PelB signal peptide and a 6X-His tag at the end of the dermaseptine CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts. Among a wide range of AMPs, polycationic dermaseptin peptides are produced by the skin glands of the frog Phyllomedusa bicolor [1]. It supplies both antibacterial and antifungal protection to a broad range of plant pathogens but shows no toxic effects on plant and mammalian cells [2]. This peptide of molecular weight of 22.1 kDa contains a tandem repeat of a chitin binding domain (CBD) as an improvement of dermaseptin-b1 gene (AKA MsrA2)(BBa_K2577001) which binds to chitin fungal cell wall, thereby increasing the lytic activity of the catalytic dom
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 392
Illegal AgeI site found at 671 - 1000COMPATIBLE WITH RFC[1000]
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