Composite

Part:BBa_K4174002:Design

Designed by: Megan Fleeharty   Group: iGEM22_William_and_Mary   (2022-10-04)
Revision as of 22:37, 4 October 2022 by Msfleeharty (Talk | contribs)

osmY-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the 2006 MIT iGEM team's composite part BBa_J45995, with GFP replaced with sfGFP from Ceroni et al. 2015, RBS BBa_B0030 replaced with an RBS containing region from Ceroni et al. 2015, scar sequences removed, and UNS1 and UNS10 added to the ends.

Source

Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. Nature Methods, 12(5):415-418. Doi: 10.1038/nmeth.3339