Composite

Part:BBa_K4387980

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-29)
Revision as of 22:05, 29 September 2022 by Registry (Talk | contribs)

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Bivalent nanobody expression with the HlyA secretion signal (VHH#2B + VHH#12B)

This composite part contains an anti-TNF nanobody construct with a myc-tag and the HlyA-tag required for the secretion with the hemolysin A secretion system (BBa_K4387979). The expression of the bivalent nanobody is under control of the inducible pBad system, followed by the T7 RBS. The myc-tag was required for the characterization of the secretion and functionality of the nanobody. The nanobody inserted in this part is the bivalent construct of nanobody VHH#2B and VHH#12B.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2055
    Illegal BglII site found at 2634
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None