Composite
Part:BBa_K4268016:Design
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-09-30)
Neck and Tail Multi-Transcriptional Unit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1760
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 398
Illegal NheI site found at 4934
Illegal PstI site found at 1760 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1760
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1760
Illegal NgoMIV site found at 6431
Illegal AgeI site found at 8462 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Golden Gate Assembly Level 2
Source
The coding sequences were derived from the S-TIP37 genome.