Composite

Part:BBa_K3247007

Designed by: Stephanie Trejo Corona   Group: iGEM19_Rice   (2019-10-16)
Revision as of 17:55, 30 September 2022 by Teodoramalacopol (Talk | contribs) (Addition of information about the part)


Para-B0034-iaaMH-L3S1P00

This cassette contains a Pcon-araC promoter, an Elowitz RBS (BBa_B0034), the genes iaaM and iaaH which together produce IAA, and a terminator. IAA (indole-3-acetic acid) or more commonly known as auxin is a plant hormone that increases root surface area and length and stimulates plant growth. There are many biosynthesis pathways to produce IAA from tryptophan, but this circuit uses two enzymes: iaaM and iaaH. The enzyme iaaM (tryptophan-2-monooxygenase) converts tryptophan into IAM (indole-3-acetamide) and the enzyme iaaH (indoleacetamide hydrolase) converts IAM into IAA (indole-3-acetic acid).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
    Illegal BamHI site found at 26
    Illegal BamHI site found at 1452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
    Illegal NgoMIV site found at 2803
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None