Part:BBa_K4140016
Cas12g
Part Description
RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.
Usage
Cas12g is a RNA-guided protein and differs from other Cas12 proteins by targeting a single strand RNA substrates Making it a potent platform for post transcription modification so we use to control PAH expression as it cleaves the mRNA of PAH at specific site without the need to recognize the PAM Sequence distinguishing it from other Cas proteins preventing the translation PAH just in case of over expression of PAH or high level of tyrosine and absence of L7Ae.
Literature Characterization
References
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1308
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 313
Illegal PstI site found at 787
Illegal PstI site found at 1729
Illegal NgoMIV site found at 1230 - 1000COMPATIBLE WITH RFC[1000]
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