![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4231018
Designed by: Shi Han Group: iGEM22_USTC (2022-09-30)
pUC19 carrying with IDI, NADH-HMG, ERG8, ERG19 and URA3 tag from Y.lipolytica
The plasmid backbone pUC19 combined with pTef1-IDI, pGAP-NADH-HMG, URA3 tag, pTef1-ERG8, pGAP-ERG19. It can be transformed to Y.lipolytica so as to establish MVA pathway.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal NheI site found at 634
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal BglII site found at 719
Illegal BglII site found at 6238
Illegal BamHI site found at 974
Illegal BamHI site found at 1073
Illegal BamHI site found at 1613
Illegal BamHI site found at 6936
Illegal BamHI site found at 8102
Illegal XhoI site found at 3028
Illegal XhoI site found at 3862
Illegal XhoI site found at 3895
Illegal XhoI site found at 6653 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2325
Illegal EcoRI site found at 8712
Illegal PstI site found at 862
Illegal PstI site found at 1992
Illegal PstI site found at 7315
Illegal NgoMIV site found at 638
Illegal NgoMIV site found at 1366
Illegal NgoMIV site found at 2247
Illegal NgoMIV site found at 2463
Illegal NgoMIV site found at 2967
Illegal NgoMIV site found at 3219
Illegal NgoMIV site found at 3531
Illegal AgeI site found at 2343
Illegal AgeI site found at 3540
Illegal AgeI site found at 3627
Illegal AgeI site found at 4153
Illegal AgeI site found at 8270 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7991
Illegal BsaI.rc site found at 2386
Illegal BsaI.rc site found at 3048
Illegal BsaI.rc site found at 3396
Illegal BsaI.rc site found at 7710
Illegal BsaI.rc site found at 8060
[edit]
Categories
Parameters
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