Composite
A1-R1-A3 p

Part:BBa_K4231016:Design

Designed by: Shi Han   Group: iGEM22_USTC   (2022-09-30)
Revision as of 16:47, 30 September 2022 by Registry (Talk | contribs)

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Y.lipolytica TEF1 promoter with a regulatory sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 797
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite biological part was constructed by overlap pcr.


Source

All parts were amplified from the genome of Yarrowia lipolytica strain W29.

References