Coding

Part:BBa_K4375006

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-28)
Revision as of 09:10, 7 October 2022 by Golaritsagnes (Talk | contribs)


Anti-CEA Nanobody (C43)

CEA-specific (carcinoembryonic antigen) Nanobody is optimized for production in E. coli.


Usage and Biology

The human carcinoembryonic antigen (CEA) is a well-studied tumor biomarkers in pancreatic cancer and is overexpressed in 83–98% of pancreatic cancers. CEA is a cell-surface glycoprotein that is normally expressed during embryogenesis, with little to no expression in normal tissue, and is used as a serum tumor marker in patients with pancreatic cancer. The Anti-CEA Nanobody recognises this tumour marker. For this reason, it is often used in a labelled form to identify tumours.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 244
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 244
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 244
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]

References

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9138244/


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Categories
Parameters
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