DNA
Part:BBa_K4162029
Designed by: Weiwen Chen Group: iGEM22_Fudan (2022-10-06)
ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP
Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2521
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2521
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2521
Illegal AgeI site found at 1018
Illegal AgeI site found at 1611 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2101
Illegal BsaI site found at 2455
[edit]
Categories
Parameters
None |