Coding

Part:BBa_K4165108

Designed by: Mennatallah Mahmoud Mohamed Abdelzaher Turky   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 20:45, 6 October 2022 by Salma Sobhy (Talk | contribs)


HTRA Binding Peptide 14

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with G of 3.8 ± 0.2 kcal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 0, molprobity = 1.62, ramachandran_favored = 100, ramachandran_outliers = 0, Qmean_4 = -5.843485, Qmean6 = -4.746417

Modeling


                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol

Docking

[edit]
Categories
Parameters
None