![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K228004
Designed by: Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
NahR( reverse) - salicylate promoter
This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
chassis | shown to work in DH5α |
copies | ~5 |
input_s | salicylate molecules |
protein | NahR |
signalling_molecule | salicylate molecules |
switch_point | 3.32μM for inducing of 2 hrs |