Regulatory

Part:BBa_K228004

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-18)
Revision as of 09:04, 18 September 2009 by Registry (Talk | contribs)

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NahR( reverse) - salicylate promoter

This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisshown to work in DH5α
copies~5
input_ssalicylate molecules
proteinNahR
signalling_moleculesalicylate molecules
switch_point3.32μM for inducing of 2 hrs