Composite
Part:BBa_K4140027
Designed by: Ahmed Gamal Mohamed Mattar Group: iGEM22_AFCM-Egypt (2022-09-28)
SEND delivery device
In this composite Lac promoter regulate the expression of phenylalanine permease that is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1045
Illegal PstI site found at 1747 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1117
Illegal NheI site found at 3526
Illegal PstI site found at 1045
Illegal PstI site found at 1747 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 240
Illegal BglII site found at 3956
Illegal BamHI site found at 477
Illegal BamHI site found at 1160
Illegal BamHI site found at 4483
Illegal XhoI site found at 3306
Illegal XhoI site found at 4193 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1045
Illegal PstI site found at 1747 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1045
Illegal PstI site found at 1747
Illegal NgoMIV site found at 3117
Illegal AgeI site found at 376 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |