Composite
PcusC-GFP
Part:BBa_K4385011:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Lead sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 462
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 462
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 462
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 462
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI.rc site found at 1394
Design Notes
This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of copper ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
Source
It is amplified from E. coli genomic DNA.