DNA

Part:BBa_K4361000

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-08-21)
Revision as of 13:51, 6 October 2022 by Larsvdb (Talk | contribs)


BlcR-binding oligo, 51 bp, scrambled


Firstly, the wildtype sequence was scrambled using the Genscript Sequence Scramble tool (https://www.genscript.com/tools/create-scrambled-sequence) to create an oligo containing the same nucleotides in a randomized order. Since this oligo does not contain either of the inverted repeat pairs, BlcR should not be able to specifically bind this molecule, thus it can be used as a negative control to compare other sequences to. This oligo was designated BBa_K4361000.


Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection
Parameters
None