Part:BBa_K4165145

TD28 Peptide

Tau binding peptide targeting the PHF seed of Tau

Usage and Biology

It is derived from D-enantiomeric peptide, its amino acid sequence is TTSLQMRLYYP. Its half maximal concentration for tau fibril formation inhibition is 7.9 μM, it can bind to tau at PHF seed VQIVYK. It can penetrate cell membrane and nuclei.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 10
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Modeling

modeled by AlphaFold2, Apptest, ITASSER, RosettaFold and TrRosetta, best model obtained from AlphaFold2

                            Figure 1.: Predicted 3D structure of Synthetic peptide TD28.

Table 1: Quality assessment parameters of TD28 model.

References

1. Dammers, C., Yolcu, D., Kukuk, L., Willbold, D., Pickhardt, M., Mandelkow, E., ... & Funke, S. A. (2016). Selection and Characterization of Tau Binding ᴅ-Enantiomeric Peptides with Potential for Therapy of Alzheimer Disease. PLoS One, 11(12), e0167432.