Plasmid

Part:BBa_K4204012:Design

Designed by: Chujing Wu   Group: iGEM22_BNDS_China   (2022-10-06)
Revision as of 11:05, 6 October 2022 by Registry (Talk | contribs)

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IPTG + sfGFP + Protein quality control system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 973
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 105
    Illegal NheI site found at 138
    Illegal NheI site found at 161
    Illegal SpeI site found at 973
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 973
    Illegal AgeI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 1047


Design Notes

Applying the QC system seamlessly connected with PART or plasmid is best.


Source

Eukaryotic translation system.

References