Composite

Part:BBa_K4321007:Design

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 00:39, 6 October 2022 by Kjanneh (Talk | contribs) (→‎Design Notes)


Cyt1Aa Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
    Illegal NotI site found at 1888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
    Illegal BglII site found at 1304
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unlike Cyt2Ba, the stability of the Cyt1Aa protein and colony formation in host and E.coli cells are reliant on the co-expression of a P20 helper protein. When trying to express Cyt1Aa, this factor should be considered.

Source

Genbank,snapgene, and addgene

References