Composite

Part:BBa_K4179013:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)
Revision as of 17:18, 5 October 2022 by Yasmin Habib (Talk | contribs) (→‎Source)


ChimeraI under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1357
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
    Illegal NgoMIV site found at 962
    Illegal AgeI site found at 1431
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.

References