Composite
Part:BBa_K4179013:Design
Designed by: Yasmin Habib Group: iGEM22_Technion-Israel (2022-10-05)
ChimeraI under Rhlr promoter + P2A + mCherry reporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2152
Illegal PstI site found at 547
Illegal PstI site found at 1792 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1357
Illegal PstI site found at 547
Illegal PstI site found at 1792 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2152
Illegal PstI site found at 547
Illegal PstI site found at 1792 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2152
Illegal PstI site found at 547
Illegal PstI site found at 1792
Illegal NgoMIV site found at 962
Illegal AgeI site found at 1431 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.
Source
test