Composite
Part:BBa_K4192114
Designed by: Lehan Dong Group: iGEM22_CAU_China (2022-09-29)
Plac-RBS-cdg-RBS-gacA
This part is used to test the effect of the interaction of cdg gene (BBa_K4192010) and gacA gene (BBa_4192011).As the most important part of promoting Pseudomonas fluorescens to produce biofilm, it has been transferred into E. coli and Pseudomonas fluorescens 2P24 for testing. We used IPTG to induce its expression, and then used crystal violet to dye 96 well plates. The results are shown in the figure below.
The effect of the two genes acting together is obviously better than that of acting alone.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 468
Illegal BamHI site found at 459
Illegal XhoI site found at 557
Illegal XhoI site found at 1001 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 392
Illegal NgoMIV site found at 1489
Illegal AgeI site found at 959 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 410
Illegal BsaI.rc site found at 977
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Categories
Parameters
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