DNA
Part:BBa_K4162028
Designed by: Weiwen Chen Group: iGEM22_Fudan (2022-10-04)
ribozyme + T7_RBS + ybbO-tdMCP-EGFP
Gene ybbO tagged with tdMCP can be combined with the stem ring structure of MS2, so that retinol dehydrogenase expressed by gene ybbO can be enriched in the area of TEARS. This part can be used to assemble polycistrons in Escherichia coli
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1612
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1612
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1612
Illegal AgeI site found at 109
Illegal AgeI site found at 702 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1192
Illegal BsaI site found at 1546
[edit]
Categories
Parameters
None |