Part:BBa_K4115043:Design

lac UV5 promoter-nifA-PnifH-sfGFP-B0015

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 82
  Illegal PstI site found at 223
  Illegal PstI site found at 1087
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 223
  Illegal PstI site found at 1087
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 724
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 82
  Illegal PstI site found at 223
  Illegal PstI site found at 1087
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 82
  Illegal PstI site found at 223
  Illegal PstI site found at 1087
  Illegal NgoMIV site found at 1344
  Illegal NgoMIV site found at 1539
  Illegal NgoMIV site found at 1614
  Illegal AgeI site found at 973
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1182
  Illegal BsaI.rc site found at 2101
  Illegal SapI site found at 405
  Illegal SapI site found at 1026
  

Design Notes

According to some papers [1], we speculate that IPTG induction experiments may be more successful in an anaerobic environment or in a medium with low nitrogen and no nitrogen.

Source

The sequence of the PhifH gene and nifA gene are gained from the genome of A.caulinodans ORS571 strain.The fragments are synthesized by the company.

References

[1] Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2