Coding
CcdA

Part:BBa_K4137000

Designed by: Clifford S Chang   Group: iGEM22_GEMS_Taiwan   (2022-08-20)
Revision as of 07:30, 9 September 2022 by Clifford (Talk | contribs)


CcdA-6xHis Antitoxin

BBa_K4137000, nicknamed ccdB for the purpose of our project, encodes for the production of CcdB protein which is a toxin to many bacteria. CcdB loses its toxicity by binding with CcdA, which prevents it from binding and interact with DNA Gyrase. The Myc tag was added to the CcdB coding sequence during the part design, this allows us to characterize the protein and go through purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None