Part:BBa_K4286099:Design
Classical oscillator composed of three genes encoding repressor protein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
First of all, our engineered microorganism will be applied to farmland, in order to prevent possible escape of engineered microorganism, the use of conditional kill switches is not necessarily a good way, because the triggering conditions in the field environment are very uncertain, which greatly increases the possibility of escape of engineered microorganism. Therefore, we conceived this auto-timing kill switch, hoping that the engineered microorganism can commit suicide at a certain time to prevent the escape. However, because Trichoderma atroviride is not a typical model organism, it lacks clearly characterized components, which is also a common problem faced by teams who want to develop new chassis, so we use the classical model organism Escherichia coli as our chassis bacteria. Because of the long experimental period, we plan to use modeling to characterize our auto-timing kill switch.
Source
This is a classical oscillator, see Ref.
References
[1]Elowitz MB, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature. 2000 Jan 20;403(6767):335-8. doi: 10.1038/35002125. PMID: 10659856. [2]Purcell O, di Bernardo M, Grierson CS, Savery NJ. A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch. PLoS One. 2011 Feb 17;6(2):e16140. doi: 10.1371/journal.pone.0016140. PMID: 21359152; PMCID: PMC3040778. [3]Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J. Synchronous long-term oscillations in a synthetic gene circuit. Nature. 2016 Oct 27;538(7626):514-517. doi: 10.1038/nature19841. Epub 2016 Oct 12. PMID: 27732583; PMCID: PMC5637407.