Coding
Part:BBa_K4323002
Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong Group: iGEM22_UManitoba (2022-09-30)
Truncated FitD
This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 909
Illegal BamHI site found at 1019
Illegal BamHI site found at 2568 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2191
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |