Coding
Part:BBa_K4388001
Designed by: Venkata Kalyan Ghadiyaram Group: iGEM22_KCL_UK (2022-09-28)
Rhodotorula glutinis Tyrosine Ammonia-Lyase Mutant (RgTAL S9N/A11T/E518V)
Rhodotorula Glutinis Tyrosine Ammonia Lyase is a gene that converts the amino acid Tyrosine into the compound P-coumaric acid.
Tyrosine Ammonia Lyase is the first enzyme in the production of multiple compounds including Naringen, resveratrol and pterostilbene. This version of the enzyme is a mutant with amino acid changes S9N,711T and E518V. These mutations have been shown to increase kCAT/km from 298 to 391 mM^-1*s^-1
The ORF of this compound was obtained from NCBI GenBank code: KF765779.1. This sequence was then codon optimized for E.coli k12; two stop codons were also added to terminate translation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2021
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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