Part:BBa_K4387007
Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Sites
"This composite part consists of the inducible PnorV_beta, sfGFP preceded by three ribosomal binding sites, a NorR regulator, and a double forward terminator. We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The increased amount of nitric oxide in the gut activates the PnorV_beta and starts the GFP expression. When the concentration of NorR exceeds the concentration of nitric oxide, the expression of NorR is inhibited till the NO concentration is high again. We wanted to further improve the construct BBa_K4387005 by adding two more ribosomal binding sites to see if we could achieve a higher GFP response. According to the data below, we could prove that the construct with two ribosomal binding sites and the codon-optimized NorR was the best and had the highest response. "
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 746
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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