![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K4115007
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-21)
NifA_upstream
This is a stable sequence located upstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 434
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 547
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 234
Illegal NgoMIV site found at 339
Illegal NgoMIV site found at 556 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 331
[edit]
Categories
Parameters
None |