Part:BBa_K4212038:Design
ChiS5
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1723
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1723
Illegal NheI site found at 1011 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1723
Illegal BglII site found at 1066
Illegal BamHI site found at 1708 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1723
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1723
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.Our dry lab team predicted 2 possible chimeras, with either CotG or CotZ attached to the chitinase. Our wet lab team attempted to verify that the CotG chimera works better than the CotZ one. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.
Source
Synthetic construct.