Composite

Part:BBa_K4115021

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-27)
Revision as of 05:53, 27 September 2022 by Registry (Talk | contribs)

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glgA gene silence

This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus by natural transformation with E. coli. The glgA sRNA in the part will transcribe small RNA fragments that can bind specifically to glgA's mRNA to inhibit the expression of glgA. The silence of glgA will inhibit G1P's formation into glycogen and promotes its formation into sucrose, which increases the synthesis of Synechococcus sucrose. This allows Synechococcus to transport more sucrose via cscB in response to E. coli carbon starvation signals.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
    Illegal PstI site found at 6912
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
    Illegal XbaI site found at 1903
    Illegal PstI site found at 6912
    Illegal AgeI site found at 2068
    Illegal AgeI site found at 6284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2065
    Illegal BsaI.rc site found at 6281


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