DNA

Part:BBa_K4115010

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 02:09, 4 October 2022 by Yurj (Talk | contribs)


NSI-US

An upstream homology arm from S.elongatus UTEX2973.

Usage and Biology

It is used together with NSI-DS (BBa_K4115011) to construct plasmids required for S.elongatus HL7942, where NSI-US is placed upstream of the aimed fragments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None