Composite
Part:BBa_K4387004
Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy Group: iGEM22_UZurich (2022-09-30)
Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002
This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP, the NorR regulator, and a double forward terminator. We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the PnorV_beta had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 742
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |