Composite

Part:BBa_K4387003

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-30)
Revision as of 15:29, 2 October 2022 by Jmehdy (Talk | contribs)


Negative Control of Nitric Oxide Sensing Genetic Circuit

To assess the proper induction of our construct by nitric oxide, we removed the pNorV_beta promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of BBa_K4387005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None