Composite

Part:BBa_K4115039:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 09:34, 3 October 2022 by Yurj (Talk | contribs) (→‎Design Notes)


J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

The function of LuxR is first verified in E.coli, which will then be used in A.caulinodans ORS570 to construct communications and regulations between E.coli and S.elongatus.

Source

The LuxR and sfGFP fragments were synthesized by the company. Plasmids are constructed by our team.

References