Coding

Part:BBa_K4115017

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-23)
Revision as of 06:34, 3 October 2022 by ParadoxDecade (Talk | contribs)


SacC (the extracellular sucrase gene)

The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase.The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.

Figure 1: Fermentation of E. coli KRXwith BBa_K863005 (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO2 at 50 %, OD600 measured every 30 minutes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal NgoMIV site found at 696
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None