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Coding
Part:BBa_K4115017
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-23)
SacC (the extracellular sucrase gene)
The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase.The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1309
Illegal PstI site found at 437 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |