Composite

Part:BBa_K4387005

Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy   Group: iGEM22_UZurich   (2022-09-30)
Revision as of 22:04, 30 September 2022 by Registry (Talk | contribs)

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Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site

This composite part consists of the inducible PnorV_beta, sfGFP preceded by one ribosomal binding site, a NorR regulator, and a double forward terminator. We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The increased amount of nitric oxide in the gut activates the PnorV_beta and starts the GFP expression. When the concentration of NorR exceeds the concentration of nitric oxide, the expression of NorR is inhibited till the NO concentration is high again.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 703
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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