Composite

Part:BBa_K4408005:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 11:48, 1 October 2022 by Xuleyu (Talk | contribs) (→‎Design Notes)


thl modified by oligopeptide SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal SapI.rc site found at 900


Design Notes

Enzyme encoded by thl catalyzes acetyl-CoA into acetoacetyl-CoA which is further transformed to 3-hydroxybutytyl-CoA by hbd encoded protein in the synthesis pathway of butyl acid in Clostridium tyrobutyricum. We designed SpyCatcher-Linker 1-thl and SpyTag-Linker 1-hbd (BBa_K4408006) to link these two enzymes together through the SpyCatcher/SpyTag system. In this way, we can improve the utilization of reagents and the reaction rate of the transformation from acetyl-CoA to 3-hydroxybutytyl-CoA, enhancing the production of butyl acid.

Source

Linker 1 is based on part BBa_K4408003 thl is based on part BBa_K3443003 SpyCatcher is based on part BBa_K3329016

References