Coding

Part:BBa_K4159000

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-13)
Revision as of 15:59, 13 July 2022 by Registry (Talk | contribs)

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GFP bindning DARPin

This part consists of the T7 promoter, RBS34, His-tag, coding sequence for a GFP-binding DARPin [1] (designed ankyrin repeat protein), and a spacer of 217bp [2]. The part does not include a terminator as the sequence has been used in ribosome display. The spacer sequence for ribosome display should preferably be about 100 amino acids in length.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None