Coding
XR

Part:BBa_K4324100:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-09-27)
Revision as of 12:01, 27 September 2022 by PenTest-duck (Talk | contribs) (References)


NAD(P)H-dependent D-xylose reductase from S. stipitis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 747


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.

Source

XYL1 gene of Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)

References

1. https://www.uniprot.org/uniprotkb/P31867/entry 2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307