Device
Part:BBa_K4408009:Design
Designed by: Leyu Xu Group: iGEM22_Worldshaper-NJBIOX (2022-09-29)
Pthl-adhE2-thl-hbd, Expression of adhE2, thl and hbd with Pthl promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3907
Illegal SapI.rc site found at 3731
Design Notes
In our program, we use this device to construct and optimize the synthesis pathways of butanol and butyrate in Clostridium tyrobutyricum. By introducing adhE2,butyryl-CoA is converted to butyl aldehyde and further transformed to butanol. Enzyme encoded by thl catalyzes acetyl-CoA into acetoacetyl-CoA which is further transformed to 3-hydroxybutytyl-CoA by hbd encoded protein. These two enzymes are key enzymes in the synthesis pathway of butyl acid in Clostridium tyrobutyricum. By overexpressing thl and hbd, we enhanced the production of butyrate in Clostridium tyrobutyricum. Pthl starts the transcription and Cpa fdx terminator ends the transcription.
Source
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