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Part:BBa_K4159003:Design

Designed by: Melissa Hendrén   Group: iGEM22_Aalto-Helsinki   (2022-07-14)
Revision as of 09:07, 14 July 2022 by Registry (Talk | contribs)

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Spacer sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No changes were needed to the sequence. This sequence was taken from the pBx1-VHH_template3-3XMyc-Spacer [1], and placed at the end of the fragment for ribosome display of the DARPin construct.


Source

[1] Chen X, Gentili M, Hacohen N, Regev A. A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies. Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z. PMID: 34535642; PMCID: PMC8448731.

References