Composite

Part:BBa_K4468011

Designed by: Zhichao Li   Group: iGEM22_HUST-China   (2022-09-30)
Revision as of 10:38, 30 September 2022 by Registry (Talk | contribs)

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T7-PmrA- T7-PmrB(FP)-T7 Terminator-PmrC-Oprf-Sitag-FP- T7 Terminator

Description This is a composite component for the absorption and recovery of rare earth elements, especially for lanthanides. It consists of T7-PmrA- T7-PmrB(FP)-T7 Terminator-PmrC-oprf-Sitag-FP- T7 Terminator. It can express PmrA and PmrB protein without any induction. Besides, lanthanide ions in the external solution can initiate the expression of oprf-Sitag-LanM protein that can achieve a large amount of lanthanide adsorption and attachment on the surface of silica column. FP protein fuses dLBT and LanM proteins together. Its adsorption efficiency and amount of lanthanides are both improved compared with ordinary LanM protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2858
    Illegal PstI site found at 1619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1619
    Illegal NgoMIV site found at 3293
    Illegal NgoMIV site found at 4127
    Illegal AgeI site found at 1813
    Illegal AgeI site found at 2642
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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