Part:BBa_K4468007
Oprf-Sitag-FP
Usage and Biology Oprf-Sitag-FP is a protein composed of oprf, Sitag, dLBT and LanM peptides. It is the main element for adsorption and recovery of lanthanides. Oprf has a membrane-binding domain, which helps the protein binding on the cell membrane of our engineered bacteria. Sitag is a tag that can connect with silicon element. It allows us to easily fix our protein just using a silica column. dLBT can highly absorb Tb3+, the most representative element of lanthanides. Thanks to HUST-China 2017, we successfully obtained the dLBT with the best adsorption effect from 12 different LBT domains. LanM has great characteristics of efficient and specific absorbing lanthanides which can effectively absorb the free lanthanides in the environment. Through GS linker to combine the first three peptides and rigid linker to combine dLBT and LanM, we have connected them as a whole and created a new protein that can stick on our E. coli membrane and fix to silica column with its engineered bacteria together. When the mining wastewater flows through the column, the lanthanides can be effectively adsorbed, so as to achieve the purpose of rare earth element recovery.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 463
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 1732
Illegal AgeI site found at 247 - 1000COMPATIBLE WITH RFC[1000]
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