Regulatory

Part:BBa_K4468001

Designed by: Zhichao Li   Group: iGEM22_HUST-China   (2022-09-30)
Revision as of 09:08, 30 September 2022 by Zhichao Li (Talk | contribs)

PgolB


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1802
    Illegal AgeI site found at 50
    Illegal AgeI site found at 399
    Illegal AgeI site found at 1337
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The GolS of Salmonella is a transcriptional regulator comes from the gold specific family MerR. Its expressed protein GolS plays a role as an operon that responsible for regulating the promoter PgolB. Upon recognizing and adsorbing extracellular Au3+, the expression of downstream genes after promoter PgolB will be activated. PgolB is the promoter of the GolS system. The expression of genes after PgolB is strictly regulated by GolS. In the absence of Au3+, GolS inhibits PgolB to prevent downstream genes’ expression. Whereas when GolS adsorbs exogenous Au3+, the inhibition turns to cease, allowing PgolB to initiate expression.

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Categories
Parameters
None