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Coding
Part:BBa_K4212009
Designed by: Shirin Bamezai Group: iGEM22_Imperial_College_London (2022-09-29)
GerAK_chimera
Synthetic operon designed with GerAA, GerKB and GerAC coding sequences from the B. subtilis genome. Designed to be transformed in a B. subtilis knockout strain so as to produce a germinant receptor that contains a binding site for glucose, and when activated triggers an orthogonal signalling cascade resulting in germination.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal NheI site found at 4
Illegal PstI site found at 533
Illegal PstI site found at 4290 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal BglII site found at 3871
Illegal XhoI site found at 640 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2384
Illegal EcoRI site found at 4339
Illegal PstI site found at 533
Illegal PstI site found at 4290 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3526
[edit]
Categories
Parameters
None |