Composite

Part:BBa_K4345004:Design

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-09-27)
Revision as of 19:59, 27 September 2022 by Registry (Talk | contribs)

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NarX mutant fused to mPapaya with a rigid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2153
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2153
    Illegal PstI site found at 659
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

NarX mutant was designed based on literature (Cavicchioli et al., 1995). mPapaya was obtained from a collegues plasmid.

References