Coding

Part:BBa_K4345009:Design

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-09-29)
Revision as of 14:00, 29 September 2022 by LVdB (Talk | contribs) (→‎Design Notes)


mNeonGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 353


Design Notes

Excitation wavelength: 506 nm Emission wavelength: 517 nm (mNeonGreen, 2022)

The sequence provided above only contains the coding sequence for mNeonGreen, no ribosomal binding site and/or promoter.

Source

This fluorescent protein is derived from Branchiostoma lanceolatum.

the sequence was obtained from the pRSETb_mNeonGreen plasmid.

References