Coding

Part:BBa_K4140022

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-28)
Revision as of 18:26, 28 September 2022 by Ahmed Mattar (Talk | contribs)


Peg10

This is a paternally expressed imprinted gene that binds its own mRNA and self-assembles into virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA. It is thought to have originated from the Ty3/Gypsy family of retrotransposons

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1117
    Illegal PstI site found at 1045
    Illegal PstI site found at 1747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal BamHI site found at 477
    Illegal BamHI site found at 1160
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1747
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None